Objectives: This study aimed to determine the relationship of breast cancer development with the polymorphisms of endothelial nitric oxide synthase (eNOS), myeloperoxidase (MPO), and uncoupling protein 2 (UCP2) genes. Methods: The study included 60 breast cancer patients and 70 healthy controls. After the exclusion criteria, 37 patients and 70 healthy controls were enrolled in the study. The functional variants examined were the intron-4 variable number of tandem repeats (VNTR), -G463A, and -866G/A variants for the eNOS, MPO, and UCP-2 genes, respectively. The polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymorphism (RFLP) methods were used for genotyping. The distribution of genotype frequencies of the eNOS, MPO, and UCP2 genes between the breast cancer patients and healthy controls was compared using the Chi-square test. Results: The BB genotype of the eNOS gene variant (intron-4 VNTR) was associated with a significantly decreased breast cancer risk (OR=0.56; 95%CI, 0.463–0.676; p=0.001); the AA and AB genotypes were not associated with the risk of breast cancer as reported in our previous work. No significant association was determined between the breast cancer risk and any genotype of the MPO gene variant. While the AA (OR=8.167; 95% CI, 2.785–23.951; p=0.001) and AG (OR=4.341; 95% CI, 1.679–11.222; p=0.002) genotypes of the UCP2 gene variant were associated with a significantly decreased breast cancer risk, GG genotype of the UCP2 gene variant was associated with a significantly increased risk (OR=5.0; 95% CI, 2.207–11.327; p=0.001). Conclusion: The Outcomes of this study revealed that breast cancer was associated with BB genotype of the intron- 4 VNTR variant of the eNOS gene and AA, AG, and GG genotypes of the -866G/A variant of the UCP2.
Corresponding Author: Dilek Erdem